標題: | Characterization of two populations of mesenchymal progenitor cells in umbilical cord blood |
作者: | Chang, YJ Tseng, CP Hsu, LF Hsieh, TB Hwang, SM 生物科技學系 Department of Biological Science and Technology |
關鍵字: | mesenchymal progenitor cells;clonogenic;differentiation |
公開日期: | 1-六月-2006 |
摘要: | Umbilical cord blood (UCB) is a valuable source for hematopoietic progenitor cell therapy. Moreover, it contains another subset of non-hematopoietic population referred to as mesenchymal progenitor cells (MPCs), which can be ex vivo expanded and differentiated into osteoblasts, chondrocytes and adipocytes. In this study, we successfully isolated the clonogenic MPCs from UCB by limiting dilution method. These cells exhibited two different morphologic phenotypes, including flattened fibroblasts (majority) and spindle-shaped fibroblasts (minority). Both types of MPCs shared similar cell surface markers except CD90 and had similar osteogenic and chondrogenic potentials. However, the spindle-shaped clones possessed the positive CD90 expression and showed a greater tendency in adipogenesis, while the flattened clones were CD90 negative cells and showed a lower tendency in adipogenesis. The high number of flattened MPCs might be linked to the less sensitivity of UCB-derived MPCs in adipogenic differentiation. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.cellbi.2005.12.009 http://hdl.handle.net/11536/12202 |
ISSN: | 1065-6995 |
DOI: | 10.1016/j.cellbi.2005.12.009 |
期刊: | CELL BIOLOGY INTERNATIONAL |
Volume: | 30 |
Issue: | 6 |
起始頁: | 495 |
結束頁: | 499 |
顯示於類別: | 期刊論文 |