單分子無標定核酸定序方法

Abstract

本發明揭露一種單分子無標定核酸定序方法，是將核酸模板與引子配對，並組裝於聚合酶上，且當核苷酸加入合成時，藉由聚合酶接合於蛋白質電晶體上所測量到的電導訊號，即能判定核酸模板之序列，此測得的電導訊號所構成之軌跡乃包含呈現明顯峰值特性的反應高原，恰可用來判別四種不同的核苷酸與對應的鹼基，並可適用於解碼各種困難的核酸序列。 A sequencing method for label-free single molecular nucleic acid is provided. The primer is used for the nucleic acid template to be assembled to a polymerase. When the nucleotides are added, the electrical conductance signal is measured by the polymerase being connected to the protein transistor to determine the sequences of the nucleic acid template. The trajectory of the measured electrical conductance signal forms a plateaux with obvious spikes, which is used to identify four kinds of the nucleotides and their bases. Furthermore, the sequencing method is suitable for sequencing of complex nucleic acids.