標題: | Detection of discriminative sequence motifs in proteins obtained from prokaryotes grown at various temperatures |
作者: | Wu, LC Horng, JT Huang, SL Huang, HD Liu, BJ 生物資訊及系統生物研究所 Institude of Bioinformatics and Systems Biology |
關鍵字: | protein thermostability;protein motif |
公開日期: | 30-四月-2006 |
摘要: | Recent investigations on the stability of proteins have demonstrated various structural factors, but few have considered sequence factors such as protein motifs. These motifs represent highly conserved regions and describe critical regions that may only exist on proteins that remain functional at high temperatures. This investigation presents a method for identifying and comparing corresponding mesophilic and thermophilic sequence motifs between protein families. Discriminative motifs that are conserved only in the mesophilic or thermophilic subfamily are identified. Analysis of the results shows that, although the subfamilies of most protein families share similar motifs, some discriminative motifs are present in particular thermophilic/mesophilic subfamilies. The thermophilic discriminative motifs are conserved only in thermophilic organisms, revealing that physiochemical principles support thermostability. (c) 2006 Wiley Periodicals, Inc. |
URI: | http://dx.doi.org/10.1002/jcc.20391 http://hdl.handle.net/11536/12354 |
ISSN: | 0192-8651 |
DOI: | 10.1002/jcc.20391 |
期刊: | JOURNAL OF COMPUTATIONAL CHEMISTRY |
Volume: | 27 |
Issue: | 6 |
起始頁: | 798 |
結束頁: | 808 |
顯示於類別: | 期刊論文 |