標題: | Crowning Proteins: Modulating the Protein Surface Properties using Crown Ethers |
作者: | Lee, Cheng-Chung Maestre-Reyna, Manuel Hsu, Kai-Cheng Wang, Hao-Ching Liu, Chia-I Jeng, Wen-Yih Lin, Li-Ling Wood, Richard Chou, Chia-Cheng Yang, Jinn-Moon Wang, Andrew H. -J. 生物資訊及系統生物研究所 Institude of Bioinformatics and Systems Biology |
關鍵字: | crown compounds;crystal growth;molecular dynamics;protein engineering;protein surfaces |
公開日期: | 24-十一月-2014 |
摘要: | Crown ethers are small, cyclic polyethers that have found wide-spread use in phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown ethers readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein-crown ether co-crystals grown in the presence of 18-crown-6. We then employed biophysical methods and molecular dynamics simulations to compare these complexes with the corresponding apoproteins and with similar complexes with ring-shaped low-molecular-weight polyethylene glycols. Our studies show that crown ethers can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions. Consequently, we propose that crown ethers can be used to modulate a wide variety of protein surface behaviors, such as oligomerization, domain-domain interactions, stabilization in organic solvents, and crystallization. |
URI: | http://dx.doi.org/10.1002/anie.201405664 http://hdl.handle.net/11536/123894 |
ISSN: | 1433-7851 |
DOI: | 10.1002/anie.201405664 |
期刊: | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
Volume: | 53 |
Issue: | 48 |
起始頁: | 13054 |
結束頁: | 13058 |
顯示於類別: | 期刊論文 |