標題: | The depletion of securin enhances butein-induced apoptosis and tumor inhibition in human colorectal cancer |
作者: | Huang, Yu-Tin Lin, Chien-I Chien, Pei-Hsuan Tang, Tsai-Tai Lin, Johnson Chao, Jui-I 生物科技學系 分子醫學與生物工程研究所 Department of Biological Science and Technology Institute of Molecular Medicine and Bioengineering |
關鍵字: | Butein;Securin;Mitosis;Apoptosis;Colorectal tumor inhibition |
公開日期: | 5-九月-2014 |
摘要: | Butein (3,4,2\',4\'-tetrahydroxychalcone) is a promising natural polyphenolic compound that shows the growth inhibitory activity in human cancer cells; however, the precise mechanism is still unclear. Securin plays pivotal role in cancer cell proliferation and tumorigenesis. Here, we report the presence of securin that could modulate apoptosis and tumor growth ability in the butein-treated human colorectal cancer. Butein induced caspase-3 activation and PARP protein cleavage for apoptosis induction in human colorectal cancer cells. Interestingly, butein reduced the securin protein levels but conversely increased the phospho-histone H3 proteins, mitotic arrest and abnormal chromosomes segregation in cancer cells. The securin-null colorectal cancer cells were more sensitive on the reduction of cell viability than the securin-wild type cancer cells following butein treatment. The loss of securin in human colorectal cancer cells decreased tumor growth ability in nude mice. Moreover, butein reduced the tumor size of xenografted human colorectal tumors of nude mice. Taken together, this study demonstrates for the first time that the depletion of securin mediates the butein-induced apoptosis and colorectal tumor inhibition. (C) 2014 Elsevier Ireland Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.cbi.2014.06.006 http://hdl.handle.net/11536/123984 |
ISSN: | 0009-2797 |
DOI: | 10.1016/j.cbi.2014.06.006 |
期刊: | CHEMICO-BIOLOGICAL INTERACTIONS |
Volume: | 220 |
起始頁: | 41 |
結束頁: | 50 |
顯示於類別: | 期刊論文 |