標題: Formation of suspended bilayer lipid membrane between electrowetting-driven encapsulated droplets
作者: Fan, Shih-Kang
Chen, Ching-Wen
Lin, Yi-Ying
Chen, Li-Chi
Tseng, Fan-Gang
Pan, Rong-Long
材料科學與工程學系
Department of Materials Science and Engineering
公開日期: 1-Sep-2014
摘要: Using limited lipid molecules held by two water-core/oil-shell encapsulated droplets, we formed an optically observable bilayer lipid membrane (BLM) across a microfabricated aperture whose dimension was precisely determined and easily scalable. For the minute volume of a 0.1 mu L oil (n-decane) shell encapsulating a 1.5 mu L water core droplet, only 0.2 to 2.8 nmol or 0.17 to 2.4 mu g lipid was required. Microscopes and electrophysiological measurements were performed on the parallel-plate device with three major steps demonstrated: (1) manipulating self-assembled lipid monolayers at the water-oil interface of the encapsulated droplets by electrowetting-on-dielectric (EWOD), (2) forming a BLM by bringing two lipid monolayers towards the aperture and thinning down the oil film between them by Young-Laplace pressure, and (3) incorporating membrane-bound nanopores, alpha-hemolysin (alpha HL), on the BLM. This study shows the influence of the lipid concentration to the interfacial tension and EWOD. Plateau-Gibbs border and black membrane area of the BLM were optically observed, while trans-membrane electrophysiological signals were electrically recorded from a pair of Ag/AgCl electrodes. (C) 2014 AIP Publishing LLC.
URI: http://dx.doi.org/10.1063/1.4896061
http://hdl.handle.net/11536/123987
ISSN: 1932-1058
DOI: 10.1063/1.4896061
期刊: BIOMICROFLUIDICS
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