Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

標題:	Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH
作者:	Deka, Gitanjal Okano, Kazunori Masuhara, Hiroshi Li, Yaw-Kuen Kao, Fu-Jen 應用化學系應用化學系分子科學碩博班光電工程學系 Department of Applied Chemistry Institute of Molecular science Department of Photonics
公開日期:	1-一月-2014
摘要:	We report a novel method for studying cellular migration in vitro. Cytophilic microdomains were formed on a cytophobic substrate by laser ablation. HeLa cells were grown on those domains until confluence, and then channels were formed to guide cellular migration. Two-photon excitation fluorescence-lifetime imaging of NADH revealed metabolic variation among migrating and nonmigrating cells.
URI:	http://dx.doi.org/10.1039/c4ra06492e http://hdl.handle.net/11536/124003
ISSN:	2046-2069
DOI:	10.1039/c4ra06492e
期刊:	RSC ADVANCES
Issue:	83
起始頁:	44100
結束頁:	44104
顯示於類別：	期刊論文

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