標題: Zinc(II) and pyrophosphate selective fluorescence probe and its application to living cell imaging
作者: Lin, Jia-Rong
Chu, Chien-Ju
Venkatesan, Parthiban
Wu, Shu-Pao
應用化學系
Department of Applied Chemistry
關鍵字: Sensors;Zn(II);Pyrophosphate;Fluorescence;Imaging agents
公開日期: 1-二月-2015
摘要: A BODIPY-based fluorescent chemosensor 1 selectively detects Zn2+ by significant fluorescence enhancement. The binding ratio of the 1-Zn2+ complex was determined from a Job plot to be 1:1. The binding constant (K-a) of Zn2+ binding to chemosensor 1 was found to be 5.9 x 10(4) M-1, with a detection limit of 0.59 mu M. Furthermore, the 1-Zn2+ complex can specifically detect pyrophosphate (PPi) through fluorescence quenching. The detection limit for detecting PPi was found to be approximately 17.5 mu M. Fluorescence microscopy imaging using RAW264.7 cells showed that chemosensor 1 can be used as an effective fluorescent probe for detecting Zn2+ and PPi in living cells. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.snb.2014.10.109
http://hdl.handle.net/11536/124034
ISSN: 0925-4005
DOI: 10.1016/j.snb.2014.10.109
期刊: SENSORS AND ACTUATORS B-CHEMICAL
Volume: 207
起始頁: 563
結束頁: 570
顯示於類別:期刊論文