標題: | Pheophytin a Inhibits Inflammation via Suppression of LPS-Induced Nitric Oxide Synthase-2, Prostaglandin E2, and Interleukin-1 beta of Macrophages |
作者: | Lin, Chun-Yu Lee, Chien-Hsing Chang, Yu-Wei Wang, Hui-Min Chen, Chung-Yi Chen, Yen-Hsu 生醫工程研究所 Institute of Biomedical Engineering |
關鍵字: | pheophytin a;NO;signal transducers and activators of transcription 1 (STAT-1);anti-inflammation |
公開日期: | 1-十二月-2014 |
摘要: | Inflammation is a serious health issue worldwide that induces many diseases such as sepsis. There has been a vast search for potentially effective drugs to decrease mortality from sepsis. Pheophytin a is a chlorophyll-related compound derived from green tea. We found that pre-treatment with pheophytin a suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E2 (PGE2), and interleukin-1 beta in RAW 264.7 macrophages. NO synthase-2 (NOS2) and cyclooxygenase-2 (COX-2) expression levels were repressed by pre-treatment with pheophytin a at both the transcriptional and translational levels. Pheophytin a inhibited NOS2 promoter activity, but not its mRNA stability, through extracellular signal-regulated kinase (ERK1/2). This suppression was reversed by ERK1/2 inhibitor (U0126). Pheophytin a reduced signal transducers and activators of transcription 1 (STAT-1) activation, without an obvious influence on activator protein-1 (AP-1) and nuclear factor kappa B (NF-kappa B). These results suggest that pheophytin a functions by down-regulating the transcriptional levels of inflammatory mediators and blocking the ERK and STAT-1 pathways. |
URI: | http://dx.doi.org/10.3390/ijms151222819 http://hdl.handle.net/11536/124096 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms151222819 |
期刊: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
Volume: | 15 |
Issue: | 12 |
起始頁: | 22819 |
結束頁: | 22834 |
顯示於類別: | 期刊論文 |