Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

Abstract

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 mu M. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.