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dc.contributor.authorSheu, Shiow-Yunnen_US
dc.contributor.authorHo, Shin-Rongen_US
dc.contributor.authorSun, Jui-Shengen_US
dc.contributor.authorChen, Ching-Yunen_US
dc.contributor.authorKe, Cherng-Jyhen_US
dc.date.accessioned2019-04-03T06:41:33Z-
dc.date.available2019-04-03T06:41:33Z-
dc.date.issued2015-01-24en_US
dc.identifier.issn1472-6882en_US
dc.identifier.urihttp://dx.doi.org/10.1186/s12906-015-0520-zen_US
dc.identifier.urihttp://hdl.handle.net/11536/124374-
dc.description.abstractBackground: In osteoarthritis (OA), the imbalance of chondrocytes' anabolic and catabolic factors can induce cartilage destruction. Interleukin-1 beta (IL-1 beta) is a potent pro-inflammatory cytokine that is capable of inducing chondrocytes and synovial cells to synthesize MMPs. The hypoxia-inducible factor-2alpha (HIF-2alpha, encoded by Epas1) is the catabolic transcription factor in the osteoarthritic process. The purpose of this study is to validate the effects of ecdysteroids (Ecd) on IL-1 beta-induced cartilage catabolism and the possible role of Ecd in treatment or prevention of early OA. Methods: Chondrocytes and articular cartilage was harvested from newborn ICR mice. Ecd effect on chondrocytes viability was tested and the optimal concentration was determined by MTT assay. The effect of HIF-2 alpha (EPAS1) in cartilage catabolism simulated by IL-1 beta (5 ng/ml) was evaluated by articular cartilage explants culture. The effects of Ecd on IL-1 beta-induced inflammatory conditions and their related catabolic genes expression were analyzed. Results: Interleukin-1 beta (IL-1 beta) treatment on primary mouse articular cartilage explants enhanced their Epas1, matrix metalloproteinases (MMP-3, MMP-13) and ADAMTS-5 genes expression and down-regulated collagen type II (Col2a1) gene expression. With the pre-treatment of 10(-8)M Ecd, the catabolic effects of IL-1 beta on articular cartilage were scavenged. Conclusion: In conclusions, Ecd can reduce the IL-1 beta-induced inflammatory effect of the cartilage. Ecd may suppress IL-1 beta-induced cartilage catabolism via HIF-2a pathway.en_US
dc.language.isoen_USen_US
dc.subject20-Hydroxyecdysoneen_US
dc.subjectIL-1 betaen_US
dc.subjectHypoxiaen_US
dc.subjectNormoxiaen_US
dc.subjectCartilage destructionen_US
dc.subjectHIF-2 alphaen_US
dc.titleArthropod steroid hormone (20-Hydroxyecdysone) suppresses IL-1 beta-induced catabolic gene expression in cartilageen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12906-015-0520-zen_US
dc.identifier.journalBMC COMPLEMENTARY AND ALTERNATIVE MEDICINEen_US
dc.citation.volume15en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department智慧型仿生系統研究中心zh_TW
dc.contributor.departmentBiomimetic Systems Research Centeren_US
dc.identifier.wosnumberWOS:000349025100001en_US
dc.citation.woscount8en_US
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