標題: Reaction-based epoxide fluorescent probe for in vivo visualization of hydrogen sulfide
作者: Sathyadevi, Palanisamy
Chen, Yu-Jen
Wu, Shou-Cheng
Chen, Yen-Hao
Wang, Yun-Ming
分子醫學與生物工程研究所
Institute of Molecular Medicine and Bioengineering
關鍵字: Fluorescein;H2S detection;Confocal imaging;Zebra fish
公開日期: 15-六月-2015
摘要: Hydrogen sulfide (H2S) has emerged as the most important biosynthetic gasotransmitters along with nitric oxide (NO) and carbon monoxide (CO). In this study, we report the design and the synthesis of a new epoxide fluorescent probe 7-glycidyloxy-9-(2-glycidyloxycarbonylphenyl)-2-xanthone (FEPO) for use in in vivo visualization of hydrogen sulfide. The probe employs a fluorescein as a fluorophore, and is equipped with an operating epoxide unit. FEPO functions via epoxide ring opening upon nucleophilic attack of H2S. This ring opening strategy may open a new avenue for the development of various H2S fluorescent sensors. FEPO showed high selectivity and high sensitivity for H2S. FEPO\'s cytotoxicity was tested using MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay. Furthermore, the use of confocal imaging of H2S and in vivo imaging in live zebra fish demonstrated FEPO\'s potential biological applications. We anticipate that, owing to their ideal properties, probes of this type will find great uses in exploring the role of H2S in biology. (C) 2015 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2015.01.070
http://hdl.handle.net/11536/124433
ISSN: 0956-5663
DOI: 10.1016/j.bios.2015.01.070
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 68
起始頁: 681
結束頁: 687
顯示於類別:期刊論文