完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lo, LC | en_US |
dc.contributor.author | Chu, CY | en_US |
dc.contributor.author | Lin, FA | en_US |
dc.contributor.author | Pan, YR | en_US |
dc.contributor.author | Li, YK | en_US |
dc.contributor.author | Lin, JJ | en_US |
dc.date.accessioned | 2014-12-08T15:17:01Z | - |
dc.date.available | 2014-12-08T15:17:01Z | - |
dc.date.issued | 2006-04-01 | en_US |
dc.identifier.issn | 0009-4536 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/12453 | - |
dc.description.abstract | Due to their industrial interest and significance, developing a rapid method for screening beta-xylosidase activities would certainly facilitate the research and improve production in xylan-related industries especially in the pulp and paper industries. Here we report the synthesis of two activity probes, LCL-6X and -12X, for beta-xylosidase. They both carry a beta-xylopyranosyl recognition head, which is linked to a latent trapping device consisting of a 2-fluoromethylphenoxyl group and a biotin reporter group. They differ only in the length of their hydrophobic linker. A model study using a beta-xylosidase from T koningii demonstrates that both probes could successfully label the target hydrolase and give rise to biotinylated proteins. They could thus potentially become a powerful tool in screening beta-xylosidase from microbial sources. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | quinone methide | en_US |
dc.subject | xylosidases | en_US |
dc.subject | activity probe | en_US |
dc.subject | xylan | en_US |
dc.subject | labeling | en_US |
dc.subject | proteome | en_US |
dc.subject | biotin | en_US |
dc.subject | mechanism-based | en_US |
dc.title | Synthesis of activity probes for beta-xylosidase | en_US |
dc.type | Article | en_US |
dc.identifier.journal | JOURNAL OF THE CHINESE CHEMICAL SOCIETY | en_US |
dc.citation.volume | 53 | en_US |
dc.citation.issue | 2 | en_US |
dc.citation.spage | 479 | en_US |
dc.citation.epage | 488 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.identifier.wosnumber | WOS:000237261100033 | - |
dc.citation.woscount | 1 | - |
顯示於類別: | 期刊論文 |