標題: | Biological imaging with nonlinear photothermal microscopy using a compact supercontinuum fiber laser source |
作者: | He, Jinping Miyazaki, Jun Wang, Nan Tsurui, Hiromichi Kobayashi, Takayoshi 電子物理學系 Department of Electrophysics |
公開日期: | 20-四月-2015 |
摘要: | Nonlinear photothermal microscopy is applied in the imaging of biological tissues stained with chlorophyll and hematoxylin. Experimental results show that this type of organic molecules, which absorb light but transform dominant part of the absorbed energy into heat, may be ideal probes for photothermal imaging without photochemical toxicity. Picosecond pump and probe pulses, with central wavelengths of 488 and 632 nm, respectively, are spectrally filtered from a compact supercontinuum fiber laser source. Based on the light source, a compact and sensitive super-resolution imaging system is constructed. Further more, the imaging system is much less affected by thermal blurring than photothermal microscopes with continuous-wave light sources. The spatial resolution of nonlinear photothermal microscopy is similar to 188 nm. It is similar to 23% higher than commonly utilized linear photothermal microscopy experimentally and similar to 43% than conventional optical microscopy theoretically. The nonlinear photothermal imaging technology can be used in the evaluation of biological tissues with high-resolution and contrast. (C) 2015 Optical Society of America |
URI: | http://dx.doi.org/10.1364/OE.23.009762 http://hdl.handle.net/11536/124669 |
ISSN: | 1094-4087 |
DOI: | 10.1364/OE.23.009762 |
期刊: | OPTICS EXPRESS |
Volume: | 23 |
Issue: | 8 |
起始頁: | 9762 |
結束頁: | 9771 |
顯示於類別: | 期刊論文 |