Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions

標題:	Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions
作者:	Wu, TK Yu, MT Liu, YT Chang, CH Wang, HJ Diau, EWG 生物科技學系應用化學系 Department of Biological Science and Technology Department of Applied Chemistry
公開日期:	30-三月-2006
摘要:	Oxidosqualene-lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants' construction of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3 beta-ol, lanosterol, and parkeol. Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps.
URI:	http://dx.doi.org/10.1021/ol053134w http://hdl.handle.net/11536/12470
ISSN:	1523-7060
DOI:	10.1021/ol053134w
期刊:	ORGANIC LETTERS
Volume:	8
Issue:	7
起始頁:	1319
結束頁:	1322
顯示於類別：	期刊論文

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