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dc.contributor.authorChiang, Chih-Hungen_US
dc.contributor.authorWu, Wai-Wahen_US
dc.contributor.authorLi, Hsin-Yangen_US
dc.contributor.authorChien, Yuehen_US
dc.contributor.authorSun, Cho-Chinen_US
dc.contributor.authorPeng, Chi-Hsienen_US
dc.contributor.authorLin, Alex Tong-Longen_US
dc.contributor.authorHuang, Chi-Shuanen_US
dc.contributor.authorLai, Ying-Hsiuen_US
dc.contributor.authorChiou, Shih-Hwaen_US
dc.contributor.authorHung, Shuen-Iuen_US
dc.contributor.authorChang, Yuh-Lihen_US
dc.contributor.authorLan, Yuan-Tzuen_US
dc.contributor.authorLiu, Dean-Moen_US
dc.contributor.authorChien, Chian-Shiuen_US
dc.contributor.authorHuo, Teh-Iaen_US
dc.contributor.authorLee, Shou-Dongen_US
dc.contributor.authorWang, Chien-Yingen_US
dc.date.accessioned2019-04-03T06:37:50Z-
dc.date.available2019-04-03T06:37:50Z-
dc.date.issued2015-01-01en_US
dc.identifier.issn0963-6897en_US
dc.identifier.urihttp://dx.doi.org/10.3727/096368915X686986en_US
dc.identifier.urihttp://hdl.handle.net/11536/124741-
dc.description.abstractAcute hepatic failure (AHF) is a severe liver injury leading to sustained damage and complications. Induced pluripotent stem cells (iPSCs) may be an alternative option for the treatment of AHF. In this study, we reprogrammed human dental pulp-derived fibroblasts into iPSCs, which exhibited pluripotency and the capacity to differentiate into tridermal lineages, including hepatocyte-like cells (iPSC-Heps). These iPSC-Heps resembled human embryonic stem cell-derived hepatocyte-like cells in gene signature and hepatic markers/functions. To improve iPSC-Heps engraftment, we next developed an injectable carboxymethyl-hexanoyl chitosan hydrogel (CHC) with sustained hepatocyte growth factor (HGF) release (HGF CHC) and investigated the hepatoprotective activity of HGF CHC-delivered iPSC-Heps in vitro and in an immunocompromised AHF mouse model induced by thioacetamide (TAA). Intrahepatic delivery of HGF CHC iPSC-Heps reduced the TAA-induced hepatic necrotic area and rescued liver function and recipient viability. Compared with PBS-delivered iPSC-Heps, the HGF CHC-delivered iPSC-Heps exhibited higher antioxidant and antiapoptotic activities that reduced hepatic necrotic area. Importantly, these HGF CHC-mediated responses could be abolished by administering anti-HGF neutralizing antibodies. In conclusion, our findings demonstrated that HGF mediated the enhancement of iPSC-Hep antioxidant/antiapoptotic capacities and hepatoprotection and that HGF CHC is as an excellent vehicle for iPSC-Hep engraftment in iPSC-based therapy against AHF.en_US
dc.language.isoen_USen_US
dc.subjectHydrogelen_US
dc.subjectInduced pluripotent stem cells (iPSCs)en_US
dc.subjectHepatocyte growth factor (HGF)en_US
dc.subjectAcute hepatic failure (AHF)en_US
dc.titleEnhanced Antioxidant Capacity of Dental Pulp-Derived iPSC-Differentiated Hepatocytes and Liver Regeneration by Injectable HGF-Releasing Hydrogel in Fulminant Hepatic Failureen_US
dc.typeArticleen_US
dc.identifier.doi10.3727/096368915X686986en_US
dc.identifier.journalCELL TRANSPLANTATIONen_US
dc.citation.volume24en_US
dc.citation.issue3en_US
dc.citation.spage541en_US
dc.citation.epage559en_US
dc.contributor.department材料科學與工程學系zh_TW
dc.contributor.departmentDepartment of Materials Science and Engineeringen_US
dc.identifier.wosnumberWOS:000352754400020en_US
dc.citation.woscount13en_US
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