標題: Stable and Efficient Paclitaxel Nanoparticles for Targeted Glioblastoma Therapy
作者: Mu, Qingxin
Jeon, Mike
Hsiao, Meng-Hsuan
Patton, Victoria K.
Wang, Kui
Press, Oliver W.
Zhang, Miqin
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: chlorotoxin;-cyclodextrin;glioblastoma;iron oxide nanoparticles;paclitaxel
公開日期: 3-六月-2015
摘要: Development of efficient nanoparticles (NPs) for cancer therapy remains a challenge. NPs are required to have high stability, uniform size, sufficient drug loading, targeting capability, and ability to overcome drug resistance. In this study, the development of a NP formulation that can meet all these challenging requirements for targeted glioblastoma multiform (GBM) therapy is reported. This multifunctional NP is composed of a polyethylene glycol-coated magnetic iron oxide NP conjugated with cyclodextrin and chlorotoxin (CTX) and loaded with fluorescein and paclitaxel (PTX) (IONP-PTX-CTX-FL). The physicochemical properties of the IONP-PTX-CTX-FL are characterized by transmission electron microscope, dynamic light scattering, and high-performance liquid chromatography. The cellular uptake of NPs is studied using flow cytometry and confocal microscopy. Cell viability and apoptosis are assessed with the Alamar Blue viability assay and flow cytometry, respectively. The IONP-PTX-CTX-FL had a uniform size of approximate to 44 nm and high stability in cell culture medium. Importantly, the presence of CTX on NPs enhanced the uptake of the NPs by GBM cells and improved the efficacy of PTX in killing both GBM and GBM drug-resistant cells. The IONP-PTX-CTX-FL demonstrated its great potential for brain cancer therapy and may also be used to deliver PTX to treat other cancers.
URI: http://dx.doi.org/10.1002/adhm.201500034
http://hdl.handle.net/11536/124777
ISSN: 2192-2640
DOI: 10.1002/adhm.201500034
期刊: ADVANCED HEALTHCARE MATERIALS
起始頁: 1236
結束頁: 1245
顯示於類別:期刊論文