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dc.contributor.authorChen, Yu-Shiunen_US
dc.contributor.authorLee, Chia-Huien_US
dc.contributor.authorHung, Meng-Yenen_US
dc.contributor.authorPan, Hsu-Anen_US
dc.contributor.authorChiou, Jin-Chernen_US
dc.contributor.authorHuang, G. Stevenen_US
dc.date.accessioned2015-07-21T08:28:53Z-
dc.date.available2015-07-21T08:28:53Z-
dc.date.issued2013-06-01en_US
dc.identifier.issn1748-3387en_US
dc.identifier.urihttp://dx.doi.org/10.1038/NNANO.2013.71en_US
dc.identifier.urihttp://hdl.handle.net/11536/124856-
dc.description.abstractThe development of personalized medicine-in which medical treatment is customized to an individual on the basis of genetic information-requires techniques that can sequence DNA quickly and cheaply. Single-molecule sequencing technologies, such as nanopores, can potentially be used to sequence long strands of DNA without labels or amplification, but a viable technique has yet to be established. Here, we show that single DNA molecules can be sequenced by monitoring the electrical conductance of a phi29 DNA polymerase as it incorporates unlabelled nucleotides into a template strand of DNA. The conductance of the polymerase is measured by attaching it to a protein transistor that consists of an antibody molecule (immunoglobulin G) bound to two gold nanoparticles, which are in turn connected to source and drain electrodes. The electrical conductance of the DNA polymerase exhibits well-separated plateaux that are similar to 3 pA in height. Each plateau corresponds to an individual base and is formed at a rate of similar to 22 nucleotides per second. Additional spikes appear on top of the plateaux and can be used to discriminate between the four different nucleotides. We also show that the sequencing platform works with a variety of DNA polymerases and can sequence difficult templates such as homopolymers.en_US
dc.language.isoen_USen_US
dc.titleDNA sequencing using electrical conductance measurements of a DNA polymerase (Retracted article. See vol. 10, pg. 563, 2015)en_US
dc.typeArticleen_US
dc.identifier.doi10.1038/NNANO.2013.71en_US
dc.identifier.journalNATURE NANOTECHNOLOGYen_US
dc.citation.spage452en_US
dc.citation.epage458en_US
dc.contributor.department材料科學與工程學系zh_TW
dc.contributor.department生物科技學系zh_TW
dc.contributor.department電控工程研究所zh_TW
dc.contributor.department生醫電子轉譯研究中心zh_TW
dc.contributor.departmentDepartment of Materials Science and Engineeringen_US
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitute of Electrical and Control Engineeringen_US
dc.contributor.departmentBiomedical Electronics Translational Research Centeren_US
dc.identifier.wosnumberWOS:000319979400018en_US
dc.citation.woscount14en_US
Appears in Collections:Articles