深層腦部光刺激黑質影響紋狀體內神經傳導物質之濃度變化

Abstract

雷射研究發展已超過五十餘年之久，雷射也衍生出許多用途，其中包含諸多臨床治療等應用。由於先前實驗中透過腦波量測已得到光刺激最佳參數，本研究目的為利用最佳光刺激參數刺激大鼠腦內之黑質緻密部，並以微透析儀抽取紋狀體的腦脊髓液來分析谷氨酸、乳酸及甘油比較其光刺激前後之濃度變化。結果，黑質緻密部在透過雷射光1 mW、5分鐘之照射下，紋狀體內的谷氨酸與乳酸濃度在光刺激後有明顯下降之現象發生，這表示對於刺激黑質緻密部可以針對紋狀體內之神經傳導物質進行調控，能有效抑制帕金森氏症或過量谷氨酸所引起之相關疾病發生。

Since the invention of the lasers more than fifty years ago, lasers have been used in a variety of biological and medical fields, including therapeutic clinical applications. From our previous research using EEG measurement, we acquired the optimal parameters for light stimulation of the active regions in the substantia nigra pars compacta (SNc). The primary goal of this thesis study is to measure the concentration changes in glutamate, lactate and glycerol in the striatum utilizing a microdialysis when the SNc is irradiated with the optimal parameters of light stimulation. The experimental results show that light stimulation with a laser power of 1 mW for 5 minutes causes the concentrations of glutamate and lactate to clearly decrease. This suggests that light stimulation of the SNc can modulate the concentrations of neurotransmitters in the striatum. The therapeutic strategy of light stimulation of the SNc for glutamate-related disease such as Parkinson’s disease is a subject that deserves serious attention in future biomedical research.