標題: Detection of foot and mouth disease and porcine reproductive and respiratory syndrome viral genes using microarray chip
作者: Liu, YC
Huang, GS
Wu, MC
Hong, MY
Hsiung, KP
材料科學與工程學系奈米科技碩博班
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
關鍵字: cDNA microarray;gene chip;foot and mouth disease virus;porcine reproductive and respiratory syndrome virus
公開日期: 1-Feb-2006
摘要: Two viral pathogens, namely, porcine reproductive and respiratory syndrome virus (PRRSV) and foot and mouth disease virus (FMDV), were selected as models for multiple pathogen detection in a cDNA microarray. Two signature regions selected from ORF2 (around 500 bp) and ORF5 (around 600 bp) of PRRVS (America serotype), and one signature region from structural genes VP1 (around 500 bp) of FMDV type O were designed and spotted on a nylon membrane. For PCR sensitivity study, the cloned FMDV-VP1 template could be diluted to near one copy and its PCR product was still detectable in gel electrophoresis. In the microarray detection, the labelling FMDV probes (3 mg/ml) could be diluted 320 times and still maintained a visible colour when hybridized with the chip. Using the mixing primers, the microarray chip demonstrated rapid and accurate detection of the specific genes. To our knowledge, this preliminary study is the first example reported applying the long signature sequences to the multiple pathogen detection in cDNA microarray.
URI: http://dx.doi.org/10.1007/s11259-006-3193-8
http://hdl.handle.net/11536/12668
ISSN: 0165-7380
DOI: 10.1007/s11259-006-3193-8
期刊: VETERINARY RESEARCH COMMUNICATIONS
Volume: 30
Issue: 2
起始頁: 191
結束頁: 204
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