Title: | An aberrant autocrine activation of the platelet-derived growth factor alpha-receptor in follicular and papillary thyroid carcinoma cell lines |
Authors: | Chen, KT Lin, JD Liou, MJ Weng, HF Chang, CA Chan, EC 生物科技學系 Department of Biological Science and Technology |
Keywords: | platelet-derived growth factor-A;platelet-derived growth factor alpha-receptor;autocrine activation;thyroid carcinoma cell line |
Issue Date: | 18-Jan-2006 |
Abstract: | Platelet-derived growth factor receptor (PDGFR) can bind to its ligand and consequently possess a kinase activity, and which is associated with the carcinogenesis of different cell types, including astrocytomas, oligodendrogliomas. and glioblastoma. In a cDNA microarray analysis, we observe the over-expressed mRNA of both PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells. And the elevated protein expressions of PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells were also confirmed by a Western blot analysis. The phosphorylation of PDGF-alpha receptor evaluated by an antibody against Tyr 720-phosphate was found in thyroid carcinoma cells. The tyrosine kinase activity of PDGF-alpha receptor was inhibited by tyrphostin AG1295 and showed a dose-dependent inhibition for the proliferation of thyroid carcinoma cells. These findings imply that autocrine activation of PDGF-alpha receptor plays a crucial role in the carcinogenesis of thyroid cells. (c) 2005 Elsevier Ireland Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.canlet.2005.01.039 http://hdl.handle.net/11536/12728 |
ISSN: | 0304-3835 |
DOI: | 10.1016/j.canlet.2005.01.039 |
Journal: | CANCER LETTERS |
Volume: | 231 |
Issue: | 2 |
Begin Page: | 192 |
End Page: | 205 |
Appears in Collections: | Articles |
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