Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Yu, Chih-Chieh | en_US |
dc.contributor.author | Corr, Christopher | en_US |
dc.contributor.author | Shen, Changyu | en_US |
dc.contributor.author | Shelton, Richard | en_US |
dc.contributor.author | Yadava, Mrinal | en_US |
dc.contributor.author | Rhea, Isaac B. | en_US |
dc.contributor.author | Straka, Susan | en_US |
dc.contributor.author | Fishbein, Michael C. | en_US |
dc.contributor.author | Chen, Zhenhui | en_US |
dc.contributor.author | Lin, Shien-Fong | en_US |
dc.contributor.author | Lopshire, John C. | en_US |
dc.contributor.author | Chen, Peng-Sheng | en_US |
dc.date.accessioned | 2015-12-02T02:59:12Z | - |
dc.date.available | 2015-12-02T02:59:12Z | - |
dc.date.issued | 2015-06-01 | en_US |
dc.identifier.issn | 1941-3149 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1161/CIRCEP.114.002296 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/127905 | - |
dc.description.abstract | Background The transmural distribution of apamin-sensitive small conductance Ca2+-activated K+ (SK) current (I-KAS) in failing human ventricles remains unclear. Methods and Results We optically mapped left ventricular wedge preparations from 12 failing native hearts and 2 rejected cardiac allografts explanted during transplant surgery. We determined transmural action potential duration (APD) before and after 100 nmol/L apamin administration in all wedges and after sequential administration of apamin, chromanol, and E4031 in 4 wedges. Apamin prolonged APD from 363 ms (95% confidence interval [CI], 341-385) to 409 (95% CI, 385-434; P<0.001) in all hearts, and reduced the transmural conduction velocity from 36 cm/s (95% CI, 30-42) to 32 cm/s (95% CI, 27-37; P=0.001) in 12 native failing hearts at 1000 ms pacing cycle length (PCL). The percent APD prolongation is negatively correlated with baseline APD and positively correlated with PCL. Only 1 wedge had M-cell islands. The percentages of APD prolongation in the last 4 hearts at 2000 ms PCL after apamin, chromanol, and E4031 were 9.1% (95% CI, 3.9-14.2), 17.3% (95% CI, 3.1-31.5), and 35.9% (95% CI, 15.7-56.1), respectively. Immunohistochemical staining of subtype 2 of SK protein showed increased expression in intercalated discs of myocytes. Conclusions SK current is important in the transmural repolarization in failing human ventricles. The magnitude of I-KAS is positively correlated with the PCL, but negatively correlated with APD when PCL is fixed. There is abundant subtype 2 of SK protein in the intercalated discs of myocytes. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | action potential | en_US |
dc.subject | calcium | en_US |
dc.subject | heart failure | en_US |
dc.subject | ion channels | en_US |
dc.subject | ventricular remodeling | en_US |
dc.title | Small Conductance Calcium-Activated Potassium Current Is Important in Transmural Repolarization of Failing Human Ventricles | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1161/CIRCEP.114.002296 | en_US |
dc.identifier.journal | CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY | en_US |
dc.citation.spage | 667 | en_US |
dc.citation.epage | 676 | en_US |
dc.contributor.department | 分子醫學與生物工程研究所 | zh_TW |
dc.contributor.department | Institute of Molecular Medicine and Bioengineering | en_US |
dc.identifier.wosnumber | WOS:000356419300023 | en_US |
dc.citation.woscount | 1 | en_US |
Appears in Collections: | Articles |