Full metadata record
DC FieldValueLanguage
dc.contributor.authorYu, Chih-Chiehen_US
dc.contributor.authorCorr, Christopheren_US
dc.contributor.authorShen, Changyuen_US
dc.contributor.authorShelton, Richarden_US
dc.contributor.authorYadava, Mrinalen_US
dc.contributor.authorRhea, Isaac B.en_US
dc.contributor.authorStraka, Susanen_US
dc.contributor.authorFishbein, Michael C.en_US
dc.contributor.authorChen, Zhenhuien_US
dc.contributor.authorLin, Shien-Fongen_US
dc.contributor.authorLopshire, John C.en_US
dc.contributor.authorChen, Peng-Shengen_US
dc.date.accessioned2015-12-02T02:59:12Z-
dc.date.available2015-12-02T02:59:12Z-
dc.date.issued2015-06-01en_US
dc.identifier.issn1941-3149en_US
dc.identifier.urihttp://dx.doi.org/10.1161/CIRCEP.114.002296en_US
dc.identifier.urihttp://hdl.handle.net/11536/127905-
dc.description.abstractBackground The transmural distribution of apamin-sensitive small conductance Ca2+-activated K+ (SK) current (I-KAS) in failing human ventricles remains unclear. Methods and Results We optically mapped left ventricular wedge preparations from 12 failing native hearts and 2 rejected cardiac allografts explanted during transplant surgery. We determined transmural action potential duration (APD) before and after 100 nmol/L apamin administration in all wedges and after sequential administration of apamin, chromanol, and E4031 in 4 wedges. Apamin prolonged APD from 363 ms (95% confidence interval [CI], 341-385) to 409 (95% CI, 385-434; P<0.001) in all hearts, and reduced the transmural conduction velocity from 36 cm/s (95% CI, 30-42) to 32 cm/s (95% CI, 27-37; P=0.001) in 12 native failing hearts at 1000 ms pacing cycle length (PCL). The percent APD prolongation is negatively correlated with baseline APD and positively correlated with PCL. Only 1 wedge had M-cell islands. The percentages of APD prolongation in the last 4 hearts at 2000 ms PCL after apamin, chromanol, and E4031 were 9.1% (95% CI, 3.9-14.2), 17.3% (95% CI, 3.1-31.5), and 35.9% (95% CI, 15.7-56.1), respectively. Immunohistochemical staining of subtype 2 of SK protein showed increased expression in intercalated discs of myocytes. Conclusions SK current is important in the transmural repolarization in failing human ventricles. The magnitude of I-KAS is positively correlated with the PCL, but negatively correlated with APD when PCL is fixed. There is abundant subtype 2 of SK protein in the intercalated discs of myocytes.en_US
dc.language.isoen_USen_US
dc.subjectaction potentialen_US
dc.subjectcalciumen_US
dc.subjectheart failureen_US
dc.subjection channelsen_US
dc.subjectventricular remodelingen_US
dc.titleSmall Conductance Calcium-Activated Potassium Current Is Important in Transmural Repolarization of Failing Human Ventriclesen_US
dc.typeArticleen_US
dc.identifier.doi10.1161/CIRCEP.114.002296en_US
dc.identifier.journalCIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGYen_US
dc.citation.spage667en_US
dc.citation.epage676en_US
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000356419300023en_US
dc.citation.woscount1en_US
Appears in Collections:Articles