Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Wen-Jui | en_US |
dc.contributor.author | Liu, Fon-Chang | en_US |
dc.contributor.author | Hsieh, Jih-Sheng | en_US |
dc.contributor.author | Chen, Ching-Hung | en_US |
dc.contributor.author | Hsiao, Shun-Yu | en_US |
dc.contributor.author | Lin, Chih-Sheng | en_US |
dc.date.accessioned | 2019-04-03T06:35:54Z | - |
dc.date.available | 2019-04-03T06:35:54Z | - |
dc.date.issued | 2015-09-04 | en_US |
dc.identifier.issn | 1477-7827 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1186/s12958-015-0099-8 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/128135 | - |
dc.description.abstract | Background: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles. Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA). Results: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate. Conclusions: The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies are needed to support this theory. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Follicular fluid | en_US |
dc.subject | in vitro fertilization | en_US |
dc.subject | Matrix metalloproteinase | en_US |
dc.subject | Oocytes maturation | en_US |
dc.subject | Tissue inhibitor of matrix metalloproteinase | en_US |
dc.title | Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1186/s12958-015-0099-8 | en_US |
dc.identifier.journal | REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY | en_US |
dc.citation.volume | 13 | en_US |
dc.citation.spage | 0 | en_US |
dc.citation.epage | 0 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000360614100003 | en_US |
dc.citation.woscount | 2 | en_US |
Appears in Collections: | Articles |
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