完整後設資料紀錄
DC 欄位語言
dc.contributor.authorYang, Wen-Juien_US
dc.contributor.authorLiu, Fon-Changen_US
dc.contributor.authorHsieh, Jih-Shengen_US
dc.contributor.authorChen, Ching-Hungen_US
dc.contributor.authorHsiao, Shun-Yuen_US
dc.contributor.authorLin, Chih-Shengen_US
dc.date.accessioned2019-04-03T06:35:54Z-
dc.date.available2019-04-03T06:35:54Z-
dc.date.issued2015-09-04en_US
dc.identifier.issn1477-7827en_US
dc.identifier.urihttp://dx.doi.org/10.1186/s12958-015-0099-8en_US
dc.identifier.urihttp://hdl.handle.net/11536/128135-
dc.description.abstractBackground: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles. Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA). Results: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate. Conclusions: The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies are needed to support this theory.en_US
dc.language.isoen_USen_US
dc.subjectFollicular fluiden_US
dc.subjectin vitro fertilizationen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectOocytes maturationen_US
dc.subjectTissue inhibitor of matrix metalloproteinaseen_US
dc.titleMatrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cyclesen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12958-015-0099-8en_US
dc.identifier.journalREPRODUCTIVE BIOLOGY AND ENDOCRINOLOGYen_US
dc.citation.volume13en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000360614100003en_US
dc.citation.woscount2en_US
顯示於類別:期刊論文


文件中的檔案:

  1. 55a40f3a50b8259a19e0e88f73acd626.pdf

若為 zip 檔案,請下載檔案解壓縮後,用瀏覽器開啟資料夾中的 index.html 瀏覽全文。