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dc.contributor.authorCheng, Chiehen_US
dc.contributor.authorTang, Meng-Cheen_US
dc.contributor.authorWu, Chung-Shuen_US
dc.contributor.authorSimon, Turibiusen_US
dc.contributor.authorKo, Fu-Hsiangen_US
dc.date.accessioned2015-12-02T02:59:31Z-
dc.date.available2015-12-02T02:59:31Z-
dc.date.issued2015-09-02en_US
dc.identifier.issn1944-8244en_US
dc.identifier.urihttp://dx.doi.org/10.1021/acsami.5b05360en_US
dc.identifier.urihttp://hdl.handle.net/11536/128288-
dc.description.abstractPeptide-based supramolecular hydrogels have been comprehensively investigated in biomaterial applications because of their unique bioactivity, biofunctionality, and biocompatible features. However, the presence of organic building blocks in peptide-based hydrogels often results in low mechanical stability. To expand their practical use and range of applications, it is necessary to develop the tool kit available to prepare bioinspired, peptide-based supramolecular hydrogels with improved mechanical stability. In this paper, we present an innovative electrostatic and cross-linking approach in which naphthyl-Phe-Phe-Cys (NapFFC) oligopeptides are combined with gold nanoparticles (AuNPs) and calcium ions (Ca2+) to produce peptide-based supramolecular hydrogels. We further investigate the interactions among NapFFC, AuNPs and Ca" by microscopy. The morphology of the nanofibrous network constructions and the binding forces exhibited from the hydrogel demonstrated that the combination of two mechanisms successfully enhanced the mechanical stability through the formation of a densely entangled fibrous network of peptide multimers that is attributed to the AuNP linkage and Ca2+-induced agglomeration. UV vis spectrophotometry and fluorescence analysis were also used to demonstrate the enhanced stability of the hydrogel under various conditions such as thermal, solvent erosion, pH value and sonication. All results indicate that the presence of AuNPs and Ca2+ can strengthen the prepared hydrogel by more than doubling the diameter of NapFFC nanofibers, enabling the formation of stronger frameworks and slowing the release of components. Further experiments confirmed that HeLa cells can grow on the bioinspired NapFFC-AuNP hydrogel and exhibit high cell viability and that these cells were killed on contact with a hydrogel containing a drug. Our peptide-based supramolecular hydrogels prepared from the observed electrostatic and cross-linking mechanisn exhibited a significantly improved mechanical stability, making them well suited to use as a drug carrier in hydrogel dressings and as extracellular materials (ECMs) for tissue engineering.en_US
dc.language.isoen_USen_US
dc.subjectgold nanoparticlesen_US
dc.subjectsupramolecularen_US
dc.subjecthydrogelen_US
dc.subjectcalcium ionsen_US
dc.subjectin vitro dressingen_US
dc.titleNew Synthesis Route of Hydrogel through A Bioinspired Supramolecular Approach: Gelation, Binding Interaction, and in Vitro Dressingen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/acsami.5b05360en_US
dc.identifier.journalACS APPLIED MATERIALS & INTERFACESen_US
dc.citation.issue34en_US
dc.citation.spage19306en_US
dc.citation.epage19315en_US
dc.contributor.department材料科學與工程學系zh_TW
dc.contributor.departmentDepartment of Materials Science and Engineeringen_US
dc.identifier.wosnumberWOS:000360868700051en_US
dc.citation.woscount1en_US
Appears in Collections:Articles