標題: When cells divide: Label-free multimodal spectral imaging for exploratory molecular investigation of living cells during cytokinesis
作者: Hsu, Jen-Fang
Hsieh, Pei-Ying
Hsu, Hsin-Yun
Shigeto, Shinsuke
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 3-Dec-2015
摘要: In vivo, molecular-level investigation of cytokinesis, the climax of the cell cycle, not only deepens our understanding of how life continues, but it will also open up new possibilities of diagnosis/prognosis of cancer cells. Although fluorescence-based methods have been widely employed to address this challenge, they require a fluorophore to be designed for a specific known biomolecule and introduced into the cell. Here, we present a label-free spectral imaging approach based on multivariate curve resolution analysis of Raman hyperspectral data that enables exploratory untargeted studies of mammalian cell cytokinesis. We derived intrinsic vibrational spectra and intracellular distributions of major biomolecular components (lipids and proteins) in dividing and nondividing human colon cancer cells. In addition, we discovered an unusual autofluorescent lipid component that appears predominantly in the vicinity of the cleavage furrow during cytokinesis. This autofluorescence signal could be utilized as an endogenous probe for monitoring and visualizing cytokinesis in vivo.
URI: http://dx.doi.org/10.1038/srep17541
http://hdl.handle.net/11536/129339
ISSN: 2045-2322
DOI: 10.1038/srep17541
期刊: SCIENTIFIC REPORTS
Volume: 5
起始頁: 0
結束頁: 0
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