標題: | Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films |
作者: | Palumbo, Fabio Camporeale, Giuseppe Yang, Yi-Wei Wu, Jong-Shinn Sardella, Eloisa Dilecce, Giorgio Calvano, Cosima Damiana Quintieri, Laura Caputo, Leonardo Baruzzi, Federico Favia, Pietro 機械工程學系 Department of Mechanical Engineering |
關鍵字: | aerosol assisted plasma;bio-composite coating;dielectric barrier discharges;lysozyme;matrix-assisted laser desorption/ionization mass spectrometry |
公開日期: | 1-十一月-2015 |
摘要: | Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 mg/cm(2) as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test. |
URI: | http://dx.doi.org/10.1002/ppap.201500039 http://hdl.handle.net/11536/129722 |
ISSN: | 1612-8850 |
DOI: | 10.1002/ppap.201500039 |
期刊: | PLASMA PROCESSES AND POLYMERS |
Volume: | 12 |
Issue: | 11 |
起始頁: | 1302 |
結束頁: | 1310 |
顯示於類別: | 期刊論文 |