標題: Metabolic engineering of cyanobacteria for the photosynthetic production of succinate
作者: Lan, Ethan I.
Wei, Crystal T.
生物科技學系
Department of Biological Science and Technology
關鍵字: Cyanobacteria;Succinic acid;Carbon dioxide re-utilization;Bio-based production
公開日期: Nov-2016
摘要: Succinate is an important commodity chemical currently used in the food, pharmaceutical, and polymer industries. It can also be chemically converted into other major industrial chemicals such as 1,4-butanediol, butadiene, and tetrahydrofuran. Here we metabolically engineered a model cyanobacterium Synechococcus elongatus PCC 7942 to photosynthetically produce succinate. We expressed the genes encoding for aketoglutarate decarboxylase and succinate semialdehyde dehydrogenase in S. elongatus PCC 7942, resulting in a strain capable of producing 120 mg/L of succinate. However, this recombinant strain exhibited severe growth retardation upon induction of the genes encoding for the succinate producing pathway, potentially due to the depletion of a-ketoglutarate. To replenish alpha-ketoglutarate, we expressed the genes encoding for phosphoenolpyruvate carboxylase and citrate synthase from Corynebacterium glutamicum into the succinate producing strain. The resulting strain successfully restored the growth phenotype and produced succinate with a titer of 430 mg/L in 8 days. These results demonstrated the possibility of photoautotrophic succinate production.
URI: http://dx.doi.org/10.1016/j.ymben.2016.10.014
http://hdl.handle.net/11536/132855
ISSN: 1096-7176
DOI: 10.1016/j.ymben.2016.10.014
期刊: METABOLIC ENGINEERING
Volume: 38
起始頁: 483
結束頁: 493
Appears in Collections:Articles