標題: | EPR Imaging Spin Probe Trityl Radical OX063: A Method for Its Isolation from Animal Effluent, Redox Chemistry of Its Quinone Methide Oxidation Product, and in Vivo Application in a Mouse |
作者: | Serda, Maciej Wu, Yen-Ku Barth, Eugene D. Halpern, Howard J. Rawal, Viresh H. 應用化學系 Department of Applied Chemistry |
公開日期: | 十二月-2016 |
摘要: | We report herein a method for the recovery, purification, and application of OX063, a costly, commercially available nontoxic spin probe widely used for electron paramagnetic resonance (EPR) imaging, as well as its corresponding quinone methide (QM) form. This precious probe can be successfully recovered after use in animal model experiments (25-47% recovery from crude lyophilizate with 98.5% purity), even from samples that are >2 years old. Significantly, the recovered trityl can be reused in further animal model EPR imaging experiments. The work also describes support for the observed formation of an air-sensitive radical derived from the QM under reducing conditions. |
URI: | http://dx.doi.org/10.1021/acs.chemrestox.6b00277 http://hdl.handle.net/11536/132992 |
ISSN: | 0893-228X |
DOI: | 10.1021/acs.chemrestox.6b00277 |
期刊: | CHEMICAL RESEARCH IN TOXICOLOGY |
Volume: | 29 |
Issue: | 12 |
起始頁: | 2153 |
結束頁: | 2156 |
顯示於類別: | 期刊論文 |