標題: Phospholamban is concentrated in the nuclear envelope of cardiomyocytes and involved in perinuclear/nuclear calcium handling
作者: Wu, Adonis Z.
Xu, Dongzhu
Yang, Na
Lin, Shien-Fong
Chen, Peng-Sheng
Cala, Steven E.
Chen, Zhenhui
分子醫學與生物工程研究所
Institute of Molecular Medicine and Bioengineering
關鍵字: Phospholamban;Calcium signaling;Cardiomyocyte;Nuclear membranes;Sarcoplasmic reticulum Ca2+ ATPase;Perinuclear Ca2+ dynamics
公開日期: 十一月-2016
摘要: Aims: Phospholamban (PLB) regulates the cardiac Ca2+-ATPase (SERCA2a) in sarcoplasmic reticulum (SR). However, the localization of PLB at subcellular sites outside the SR and possible contributions to Ca2+ cycling remain unknown. We examined the intracellular distribution of PLB and tested whether a pool of PLB exists in the nuclear envelope (NE) that might regulate perinuclear/nuclear Ca2+ (nCa(2+)) handling in cardiomyocytes (CMs). Methods and results: Using confocal immunofluorescence microscopy and immunoblot analyses of CMs and CM nuclei, we discovered that PLB was highly concentrated in NE. Moreover, the ratio of PLB levels to SERCA levels was greater in NE than in SR. The increased levels of PLB in NE were a consistent finding using a range of antibodies, tissue samples, and species. To address a possible role in affecting Ca2+ handling, we used Fluo-4 based con focal Ca2+ imaging, with scan-lines across cytosol and nuclei, and evaluated the effects of PLB on cytosolic and nCa(2+) uptake and release in mouse CMs. In intact CMs, isoproterenol increased amplitude and decreased the decay time of Ca2+ transients not only in cytosol but also in nuclear regions. In saponin-permeabilized mouse CMs ([Ca2+]; = 400 nM), we measured spontaneous Ca2+ waves after specific reversal of PLB activity by addition of the Fab fragment of an anti-PLB monoclonal antibody (100 mu g/ml).This highly selective immunological reagent enhanced Ca2+ uptake (faster decay times) and Ca2+ release (greater intensity) in both cytosol and across the nuclear regions. Conclusions: Besides SR, PLB is concentrated in NE of CMs, and may be involved in modulation of nCa(2+) dynamics. (C) 2016 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.yjmcc.2016.09.008
http://hdl.handle.net/11536/133003
ISSN: 0022-2828
DOI: 10.1016/j.yjmcc.2016.09.008
期刊: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
Volume: 100
起始頁: 1
結束頁: 8
顯示於類別:期刊論文