標題: | Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis |
作者: | Lin, Yan-Ren Li, Chao-Jui Syu, Shih-Han Wen, Cheng-Hao Buddhakosai, Waradee Wu, Han-Ping Chen, Cheng Hsu Lu, Huai-En Chen, Wen-Liang 生物科技學系 Department of Biological Science and Technology |
公開日期: | 1-一月-2016 |
摘要: | Postcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and cell models. Forty Wistar rats with postcardiac arrest acidosis (blood pH < 7.2) were included. They were divided into study (500 mg/kg L-alanyl-L-glutamine, n = 20) and control (normal saline,n = 20) groups. Each of the rats received resuscitation. The outcomes were compared between the two groups. In addition, cardiomyocytes derived from human induced pluripotent stem cells were exposed to HBSS with different pH levels (7.3 or 6.5) or to culture medium (control). Apoptosis-related markers and beating function were analyzed. We found that the duration of survival was significantly longer in the study group (n < 0.05). In addition, in pH 6.5 or pH 7.3 HBSS buffer, the expression levels of cell stress (p53) and apoptosis (caspase-3, Bcl-xL) markers were significantly lower in cardiomyocytes treated with 50 mM L-glutamine than those without L-glutamine (RT-PCR). L-glutamine also increased the beating function of cardiomyocytes, especially at the lower pH level (6.5). More importantly, glutamine decreased cardiomyocyte apoptosis and increased these cells' beating function at a low pH level. |
URI: | http://dx.doi.org/10.1155/2016/2106342 http://hdl.handle.net/11536/133011 |
ISSN: | 2314-6133 |
DOI: | 10.1155/2016/2106342 |
期刊: | BIOMED RESEARCH INTERNATIONAL |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 期刊論文 |