完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chen, Wei-Yen | en_US |
dc.contributor.author | Lin, Chia-Lung | en_US |
dc.contributor.author | Chuang, Jen-Hua | en_US |
dc.contributor.author | Chiu, Fu-Yu | en_US |
dc.contributor.author | Sun, Yun-Ya | en_US |
dc.contributor.author | Liang, Mei-Chih | en_US |
dc.contributor.author | Lin, Yenshou | en_US |
dc.date.accessioned | 2019-04-03T06:37:06Z | - |
dc.date.available | 2019-04-03T06:37:06Z | - |
dc.date.issued | 2017-01-20 | en_US |
dc.identifier.issn | 2045-2322 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1038/srep41159 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/133028 | - |
dc.description.abstract | Mammalian target of rapamycin (mTOR) plays a range of crucial roles in cell survival, growth, proliferation, metabolism, and morphology. However, mTOR forms two distinct complexes, mTOR complex 1 and mTOR complex 2 (mTORC1 and mTORC2), via association with a series of different components; this allows the complexes to execute their wide range of functions. This study explores further the composition of the mTORC2 complex. Utilizing Rictor knock-out cells, immunoprecipitation and mass spectrometry, a novel Rictor associated protein, heterogeneous nuclear ribonucleoprotein M (hnRNP M), was identified. The association between hnRNP M and Rictor was verified using recombinant and endogenous protein and the binding site was found to be within aa 1 similar to 532 of hnRNP M. The presence of hnRNP M significantly affects phosphorylation of SGK1 S422, but not of Akt S473, PKC alpha S657 and PKC zeta T560. Furthermore, hnRNP M also plays a critical role in muscle differentiation because knock-down of either hnRNP M or Rictor in C2C12 myoblasts reduced differentiation. This decrease is able to be rescued by overexpression SGK S422D in hnRNP M knockdown C2C12 myoblasts. Taken together, we have identified a novel Rictor/mTOR binding molecule, hnRNP M, that allows mTORC2 signaling to phosphorylate SGK1 thus regulating muscle differentiation. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Heterogeneous nuclear ribonucleoprotein M associates with mTORC2 and regulates muscle differentiation | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1038/srep41159 | en_US |
dc.identifier.journal | SCIENTIFIC REPORTS | en_US |
dc.citation.volume | 7 | en_US |
dc.citation.spage | 0 | en_US |
dc.citation.epage | 0 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000392378900001 | en_US |
dc.citation.woscount | 4 | en_US |
顯示於類別: | 期刊論文 |