標題: Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy
作者: Noothalapati, Hemanth
Uemura, Suguru
Ohshima, Naoki
Kinoshita, Yoshikazu
Ando, Masahiro
Hamaguchi, Hiro-o
Yamamoto, Tatsuyuki
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Raman spectroscopy;Eosinophilic esophagitis;Non-bioptic diagnosis;Eosinophil peroxidase
公開日期: 七月-2016
摘要: Eosinophilic esophagitis (EoE) is believed to be a rare pathological condition that is characterized by dense infiltration of eosinophils in esophageal epithelial layer. Occurrence of this disease worldwide has started increasing rapidly in the last decade. Routine endoscopic observations can help in diagnosis only if clear longitudinal furrows or multiple concentric rings are observed but does not give any definitive conclusion in the early stages. Hence esophageal tissue samples are collected from multiple sites by biopsy and the number of eosinophils is counted after staining. Such a procedure is time consuming and has an inherent risk of bleeding, eventually damaging esophagus. Hence we developed a resonance Raman spectroscopy based approach to detect eosinophils in esophagus using mouse models. Our results show the presence of eosinophils in mice esophageal tissues suffering from inflammation by administering interleukin-33 while there are none in control mice. We believe this method can be used in clinical application for diagnosing EoE in the near future. (C) 2016 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.vibspec.2016.03.016
http://hdl.handle.net/11536/133886
ISSN: 0924-2031
DOI: 10.1016/j.vibspec.2016.03.016
期刊: VIBRATIONAL SPECTROSCOPY
Volume: 85
起始頁: 7
結束頁: 10
顯示於類別:期刊論文