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dc.contributor.authorHung, Chih-Changen_US
dc.contributor.authorYabushita, Atsushien_US
dc.contributor.authorKobayashi, Takayoshien_US
dc.contributor.authorChen, Pei-Fengen_US
dc.contributor.authorLiang, Keng S.en_US
dc.date.accessioned2017-04-21T06:56:35Z-
dc.date.available2017-04-21T06:56:35Z-
dc.date.issued2016-07-08en_US
dc.identifier.issn0301-4622en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bpc.2016.05.001en_US
dc.identifier.urihttp://hdl.handle.net/11536/133896-
dc.description.abstractUltrafast transient absorption spectroscopy of endothelial NOS oxygenase domain (eNOS-oxy) was performed to study dynamics of ligand or substrate interaction under Soret band excitation. Photo-excitation dissociates imidazole ligand in <300 fs, then followed by vibrational cooling and recombination within 2 ps. Such impulsive bond breaking and late rebinding generate proteinquakes, which relaxes in several tens of picoseconds. The photo excited dynamics of eNOS-oxy with L-arginine substrate mainly occurs at the local site of heme, including ultrafast internal conversion within 400 fs, vibrational cooling, charge transfer, and complete ground-state recovery within 1.4 ps. The eNOS-oxy without additive is partially bound with water molecule, thus its photoexcited dynamics also shows ligand dissociation in <800 fs. Then it followed by vibrational cooling coupled with charge transfer in 4.8 ps, and recombination of ligand to distal side of heme in 12 ps. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectUltrafast spectroscopyen_US
dc.subjectNitric oxide synthaseen_US
dc.subjectPhoto-dissociationen_US
dc.subjectLigand rebindingen_US
dc.titleUltrafast dynamics of ligand and substrate interaction in endothelial nitric oxide synthase under Soret excitationen_US
dc.identifier.doi10.1016/j.bpc.2016.05.001en_US
dc.identifier.journalBIOPHYSICAL CHEMISTRYen_US
dc.citation.volume214en_US
dc.citation.spage11en_US
dc.citation.epage16en_US
dc.contributor.department電子物理學系zh_TW
dc.contributor.department物理研究所zh_TW
dc.contributor.departmentDepartment of Electrophysicsen_US
dc.contributor.departmentInstitute of Physicsen_US
dc.identifier.wosnumberWOS:000378184800002en_US
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