Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Tseng, Chin-Kai | en_US |
dc.contributor.author | Lin, Chun-Kuang | en_US |
dc.contributor.author | Wu, Yu-Hsuan | en_US |
dc.contributor.author | Chen, Yen-Hsu | en_US |
dc.contributor.author | Chen, Wei-Chun | en_US |
dc.contributor.author | Young, Kung-Chia | en_US |
dc.contributor.author | Lee, Jin-Ching | en_US |
dc.date.accessioned | 2019-04-03T06:40:07Z | - |
dc.date.available | 2019-04-03T06:40:07Z | - |
dc.date.issued | 2016-08-24 | en_US |
dc.identifier.issn | 2045-2322 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1038/srep32176 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/134078 | - |
dc.description.abstract | Dengue virus (DENV) infection and replication induces oxidative stress, which further contributes to the progression and pathogenesis of the DENV infection. Modulation of host antioxidant molecules may be a useful strategy for interfering with DENV replication. In this study, we showed that induction or exogenous overexpression of heme oxygenase-1 (HO-1), an antioxidant enzyme, effectively inhibited DENV replication in DENV-infected Huh-7 cells. This antiviral effect of HO-1 was attenuated by its inhibitor tin protoporphyrin (SnPP), suggesting that HO-1 was an important cellular factor against DENV replication. Biliverdin but not carbon monoxide and ferrous ions, which are products of the HO-1 on heme, mediated the HO-1-induced anti-DENV effect by non-competitively inhibiting DENV protease, with an inhibition constant (Ki) of 8.55 +/- 0.38 mu M. Moreover, HO-1 induction or its exogenous overexpression, rescued DENV-suppressed antiviral interferon response. Moreover, we showed that HO-1 induction by cobalt protoporphyrin (CoPP) and andrographolide, a natural product, as evidenced by a significant delay in the onset of disease and mortality, and virus load in the infected mice's brains. These findings clearly revealed that a drug or therapy that induced the HO-1 signal pathway was a promising strategy for treating DENV infection. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Human heme oxygenase 1 is a potential host cell factor against dengue virus replication | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1038/srep32176 | en_US |
dc.identifier.journal | SCIENTIFIC REPORTS | en_US |
dc.citation.volume | 6 | en_US |
dc.citation.spage | 0 | en_US |
dc.citation.epage | 0 | en_US |
dc.contributor.department | 生醫工程研究所 | zh_TW |
dc.contributor.department | Institute of Biomedical Engineering | en_US |
dc.identifier.wosnumber | WOS:000381853800001 | en_US |
dc.citation.woscount | 17 | en_US |
Appears in Collections: | Articles |
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