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dc.contributor.authorChiu, Hsi-Hoen_US
dc.contributor.authorHsieh, Yin-Chengen_US
dc.contributor.authorChen, Ya-Hueien_US
dc.contributor.authorWang, Hsin-Yingen_US
dc.contributor.authorLu, Chia-Yuen_US
dc.contributor.authorChen, Chun-Jungen_US
dc.contributor.authorLi, Yaw-Kuenen_US
dc.date.accessioned2017-04-21T06:55:15Z-
dc.date.available2017-04-21T06:55:15Z-
dc.date.issued2016-10en_US
dc.identifier.issn0175-7598en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s00253-016-7536-2en_US
dc.identifier.urihttp://hdl.handle.net/11536/134209-
dc.description.abstractGlycosyltransferase-1 from Bacillus cereus (BcGT1) catalyzes a reaction that transfers a glucosyl moiety to flavonoids, such as quercetin, kaempferol, and myricetin. The enzymatic glucosidation shows a broad substrate specificity when the reaction is catalyzed by wild-type BcGT1. Preliminary assays demonstrated that the F240A mutant significantly improves the regioselectivity of enzymatic glucosidation toward quercetin. To unveil and further to control the catalytic function of BcGT1, mutation of F240 to other amino acids, such as C, E, G, R, Y, W, and K, was performed. Among these mutants, F240A, F240G, F240R, and F240K greatly altered the regioselectivity. The quercetin-3-O-glucoside, instead of quercetin-7-O-glucoside as for the wild-type enzyme, was obtained as the major product. Among these mutants, F240R showed nearly 100 % product specificity but only retained 25 % catalytic efficiency of wild-type enzyme. From an inspection of the protein structure, we found two other amino acids, F132 and F138, together with F240, are likely to form a hydrophobic binding region, which is sufficiently spacious to accommodate substrates with varied aromatic moieties. Through the replacement of a phenylalanine by a tyrosine residue in the substrate-binding region, the mutants may be able to fix the orientation of flavonoids, presumably through the formation of a hydrogen bond between substrates and mutants. Multiple mutants-F240R_F132Y, F240R_F138Y, and F240R_F132Y_F138Y-were thus constructed for further investigation. The multiple points of mutants not only maintained the high product specificity but also significantly improved the catalytic efficiency, relative to F240R. The same product specificity was obtained when kaempferol and myricetin were used as a substrate.en_US
dc.language.isoen_USen_US
dc.subjectGlucosyltransferaseen_US
dc.subjectBcGT1en_US
dc.subjectFlavonoiden_US
dc.subjectRegioselectivityen_US
dc.subjectGlucosidationen_US
dc.titleThree important amino acids control the regioselectivity of flavonoid glucosidation in glycosyltransferase-1 from Bacillus cereusen_US
dc.identifier.doi10.1007/s00253-016-7536-2en_US
dc.identifier.journalAPPLIED MICROBIOLOGY AND BIOTECHNOLOGYen_US
dc.citation.volume100en_US
dc.citation.issue19en_US
dc.citation.spage8411en_US
dc.citation.epage8424en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000383242500013en_US
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