可調式電阻脈衝感測(TRPS)結合寡核苷酸修飾金奈米探針應用於即時免標定之多重抗菌劑檢測

Abstract

可調式電阻脈衝偵測法(TRPS)由於能夠應用於免標定分析，因此已迅速成為一新型的生物感測技術。我們利用寡核苷酸所修飾的金奈米粒子作為探針來偵測兩種常見的抗菌物質: 銀離子和可喃因。以此作為分析模型，建立一個可同時且即時定量金屬離子和小分子藥物的分子診斷方法。本研究中藉由選用適當大小的奈米孔徑試片以及所設計之不同序列核苷酸，可以調控金奈米粒子的聚集反應使分析系統最佳化。目前實驗結果已顯示，此可調式電阻脈衝感測方法可應用多參數分析技術的開發，我們並期待其未來應用在快速即時、免標定原位水相分析的潛力。

Tunable resistive pulse sensing (TRPS) has emerged owing to the feasibility for label-free analysis and has been undergoing rapid development for biosensing. Here we employed the oligonucleotide-modified gold nanoparticle (AuNP) as the detection probes to enable simultaneous measurement of two known antibiotic agents: silver ion and coralyne, which served as a practical molecular diagnostic model for concurrent real-time metal ions and small compounds exposure analysis. Various oligonucleotide sequences design and the spacer had been examined to optimize these AuNP-based probes, manipulating the analyted-triggered aggregation reaction for TRPS at appropriate nanopore size. This study has demonstrated the feasibility of TRPS system for multiplex assay, and we foresee its future potential for rapid real-time and label-free in situ water monitoring.