利用氧化石墨烯及鈦酸鹽奈米管增強厭氧氨氧化菌活性之研究

Abstract

Nitrogen removal from wastewater is gaining worldwide attention because of the potential threat of nitrogen species to the environment. Anaerobic ammonium oxidation (Anammox) is a promising method which can convert ammonium ion directly to nitrogen gas. However, Anammox still faces the difficulty in the enhancement of slow growth rate of Anammox microorganisms. Herein, we have systematically investigated the effect of graphene oxide (GO) and titanium nanotube (TNTs) on the Anammox biomass for nitrogen removal. TNTs, the 1- dimensional negatively charged nanomaterials show little effect on the enhancement of Anammox growth, presumably attributed to the strong repulsive force between TNTs and Annamox bacteria. GO makes a remarkable impact on the bacterial growth. After the batch incubation of 5h, the Anammox activity in the presence of 0.1 g/L GO is enhanced 14.2 % when compares with that in the absence of nanomaterial. The continuous experiment also proves the applicability of using GO as an effective support to improve nitrogen removal. GO utilization can enhance Anammox activity by 30% compares with that of the normal reactor after 113d of incubation. In the GO induced reactor, the effluent concentrations of ammonium and nitrite decrease steadily and reach the steady state after 80d of incubation, while it takes 127 d in the reactor without GO to achieve the steady state. These results clearly demonstrate the feasibility of utilizing GO as the support for Anammox bacteria to shorten the start-up incubation time by rapid acclimation as well as to enhance the activity of Anammox bacteria.

Nitrogen removal from wastewater is gaining worldwide attention because of the potential threat of nitrogen species to the environment. Anaerobic ammonium oxidation (Anammox) is a promising method which can convert ammonium ion directly to nitrogen gas. However, Anammox still faces the difficulty in the enhancement of slow growth rate of Anammox microorganisms. Herein, we have systematically investigated the effect of graphene oxide (GO) and titanium nanotube (TNTs) on the Anammox biomass for nitrogen removal. TNTs, the 1- dimensional negatively charged nanomaterials show little effect on the enhancement of Anammox growth, presumably attributed to the strong repulsive force between TNTs and Annamox bacteria. GO makes a remarkable impact on the bacterial growth. After the batch incubation of 5h, the Anammox activity in the presence of 0.1 g/L GO is enhanced 14.2 % when compares with that in the absence of nanomaterial. The continuous experiment also proves the applicability of using GO as an effective support to improve nitrogen removal. GO utilization can enhance Anammox activity by 30% compares with that of the normal reactor after 113d of incubation. In the GO induced reactor, the effluent concentrations of ammonium and nitrite decrease steadily and reach the steady state after 80d of incubation, while it takes 127 d in the reactor without GO to achieve the steady state. These results clearly demonstrate the feasibility of utilizing GO as the support for Anammox bacteria to shorten the start-up incubation time by rapid acclimation as well as to enhance the activity of Anammox bacteria.