添加亞鐵離子對於厭氧氨氧化程序之影響

Abstract

作为培养Anammox细菌所必需的必需营养物质，Fe（II）有望影响Anammox细菌的生长和活性。在本研究中，我们调查了Fe（II）的短期（急性效应）和长期（慢性作用）对厌氧氧化活性的影响。在短期研究中，结果表明，通过改善约60％的特异性厌氧性活性（SAA）（与对照试验相比，0.00mM），Fe（II）对阿尼莫斯的最佳浓度为0.08mM。发现Fe（II）的IC50为0.192mM。基于批次试验的结果估算Anammox比生长速度的动力学，并用Han-Levenspiel的底物抑制动力学模型进行评价。 Han-Levenspiel模型预测的Fe（II）的最佳浓度和IC50分别与批次试验相同，分别为0.07mM和0.20mM。评估了Fe（II）对测序间歇反应器（SBR）性能的长期影响。结果显示，当添加0.08mM的Fe（II）时，适当的Fe（II）添加增强了达到85％NH4 + -N和96％NO2-N去除效率的Anammox活性，同时实现了82％的NH4 + -N和87％当规定Fe（II）浓度（0.04mM）时，NO2-N去除效率。与批次试验相比，SBR系统显示出对Fe（II）（0.20mM）的IC50的抗性，并且没有引起50％的抑制。在恢复状态下，反应堆的性能仍然要抑制一个多月。 39天后，系统开始恢复，表明SBR过程的微生物群落适应性良好，达到87％的NH4 + -N和95％的NO2-N去除效率

As an essential nutrient that required in cultivating Anammox bacteria, Fe (II) is expected to affect the growth and activity of Anammox bacteria. In this study, we investigate both short-term (acute effect) and long-term (chronic effect) of Fe (II) on Anammox activity. In the short-term study, results demonstrated that the optimum concentration of Fe (II) on anammox is 0.08 mM by improving Specific Anammox Activity (SAA) approximately 60% (compared to control assay, 0.00 mM). The IC50 of Fe (II) was found to be 0.192 mM. Kinetics of Anammox specific growth rate was estimated based on results of the batch test and was evaluated with Han-Levenspiel’s substrate inhibition kinetics model. The optimum concentration and IC50 of Fe (II) predicted by Han-Levenspiel model are similar to the batch test, with values of 0.07 mM and 0.20 mM respectively. The long-term effect of Fe (II) on the performance of Sequencing batch reactor (SBR) was evaluated. Results evidently show that appropriate Fe (II) addition enhance Anammox activity achieving 85% NH4⁺-N and 96% NO2‾-N removal efficiency when 0.08 mM of Fe (II) was added while achieving 82% NH4⁺-N and 87% NO2‾-N removal efficiency when regular Fe (II) concentration (0.04 mM) was added. The SBR system had shown resistance to IC50 of Fe (II) (0.20 mM) with a relatively higher performance compared to batch test and did not cause 50% inhibition. During recovery state, the reactor performance remains to suppress for more than one month. After 39 days, the system starts to recover, suggesting the good acclimatization of microbial communities of SBR process achieving 87% NH4⁺-N and 95% NO2‾-N removal efficiency.