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dc.contributor.authorLin, Chien Y.en_US
dc.contributor.authorHuang, Jung Y.en_US
dc.contributor.authorLo, Leu-Weien_US
dc.date.accessioned2019-04-03T06:36:30Z-
dc.date.available2019-04-03T06:36:30Z-
dc.date.issued2017-02-01en_US
dc.identifier.issn1424-8220en_US
dc.identifier.urihttp://dx.doi.org/10.3390/s17020315en_US
dc.identifier.urihttp://hdl.handle.net/11536/144131-
dc.description.abstractCell-penetrating peptides (CPPs) can translocate across cell membranes, and thus have great potential for the cellular delivery of macromolecular cargoes. However, the mechanism of this cellular uptake process is not yet fully understood. In this study, a time-lapse single-particle light-sheet microscopy technique was implemented to obtain a parallel visualization of the translocating process of individual human immunodeficiency virus 1 (HIV-1) transactivator of transcription (Tat) peptide conjugated quantum dots (TatP-QDs) in complex cellular terrains. Here, TatP-QDs served as nanoscale dynamic pens, which depict remarkable trajectory aggregates of TatP-QDs on the cell surface. Spectral-embedding analysis of the trajectory aggregates revealed a manifold formed by isotropic diffusion and a fraction of directed movement, possibly caused by interaction between the Tat peptides and heparan sulfate groups on the plasma membrane. Further analysis indicated that the membrane deformation induced by Tat-peptide attachment increased with the disruption of the actin framework in cytochalasin D (cyto D)-treated cells, yielding higher interactions on the TatP-QDs. In native cells, the Tat peptides can remodel the actin framework to reduce their interaction with the local membrane environment. Characteristic hot spots for interaction were detected on the membrane, suggesting that a funnel passage may have formed for the Tat-coated particles. This finding offers valuable insight into the cellular delivery of nanoscale cargo, suggesting an avenue for direct therapeutic delivery.en_US
dc.language.isoen_USen_US
dc.subjectsingle-particle trackingen_US
dc.subjectcell-penetrating peptidesen_US
dc.subjectheparan sulfate proteoglycanen_US
dc.subjectactin filamentsen_US
dc.subjectlive cellen_US
dc.subjectplasma membraneen_US
dc.titleDepicting Binding-Mediated Translocation of HIV-1 Tat Peptides in Living Cells with Nanoscale Pens of Tat-Conjugated Quantum Dotsen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/s17020315en_US
dc.identifier.journalSENSORSen_US
dc.citation.volume17en_US
dc.citation.issue2en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department田家炳光電中心zh_TW
dc.contributor.departmentTin Ka Ping Photonics Centeren_US
dc.identifier.wosnumberWOS:000395482700099en_US
dc.citation.woscount0en_US
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