標題: | Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor |
作者: | Tsai, Meng-Zhe Hsiung, Chan-Te Chen, Yang Huang, Cheng-Sheng Hsu, Hsin-Yun Hsieh, Pei-Ying 機械工程學系 應用化學系 Department of Mechanical Engineering Department of Applied Chemistry |
公開日期: | 21-一月-2018 |
摘要: | We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL(-1) for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples. |
URI: | http://dx.doi.org/10.1039/c7an01374d http://hdl.handle.net/11536/144368 |
ISSN: | 0003-2654 |
DOI: | 10.1039/c7an01374d |
期刊: | ANALYST |
Volume: | 143 |
起始頁: | 503 |
結束頁: | 510 |
顯示於類別: | 期刊論文 |