標題: Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor
作者: Tsai, Meng-Zhe
Hsiung, Chan-Te
Chen, Yang
Huang, Cheng-Sheng
Hsu, Hsin-Yun
Hsieh, Pei-Ying
機械工程學系
應用化學系
Department of Mechanical Engineering
Department of Applied Chemistry
公開日期: 21-一月-2018
摘要: We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL(-1) for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.
URI: http://dx.doi.org/10.1039/c7an01374d
http://hdl.handle.net/11536/144368
ISSN: 0003-2654
DOI: 10.1039/c7an01374d
期刊: ANALYST
Volume: 143
起始頁: 503
結束頁: 510
顯示於類別:期刊論文