標題: Generation and Characterization of Antinonstructural Protein 1 Monoclonal Antibodies and Development of Diagnostics for Dengue Virus Serotype 2
作者: Tang, Yin-Liang
Liu, I-Ju
Li, Pi-Chun
Chiu, Chien-Yu
Lin, Chun-Yu
Huang, Chung-Hao
Chen, Yen-Hsu
Fu, Chi-Yu
Chao, Day-Yu
King, Chwan-Chuen
Wu, Han-Chung
生物科技學系
Department of Biological Science and Technology
公開日期: 1-Jan-2017
摘要: Dengue virus (DENV) circulates in tropical and subtropical areas around the world, where it causes high morbidity and mortality. There is no effective treatment of infection, with supportive care being the only option. Furthermore, early detection and diagnosis are important to facilitate clinical decisions. In this study, seven monoclonal antibodies (mAbs) recognizing nonstructural protein 1 (NS1) of DENV were generated by hybridoma techniques. These antibodies can be divided into two groups: serotype-specific (DB6-1, DB12-3, and DB38-1) and nonspecific (consisting of antibodies DB16-1, DB20-6, DB29-1, and DB41-2). The B-cell epitopes of DB20-6 and DB29-1 were identified by phage display and site-directed mutagenesis, and its binding motif, WXXWGK, was revealed to correspond to amino acid residues 115-120 of the DENV-2 NS1 protein. A diagnostic platform, consisting of a serotype-specific capture antibody and a complex detection antibody, exhibited a detection limit of about 1 ng/mL, which is sufficient to detect NS1 in clinical serum samples from dengue patients. This diagnostic platform displayed better specificity and sensitivity than two examined commercial NS1 diagnostic platforms. In summary, our results indicate that these newly generated mAbs are suitable for detection of NS1 protein of DENV-2 in clinical samples.
URI: http://dx.doi.org/10.4269/ajtmh.17-0003
http://hdl.handle.net/11536/144414
ISSN: 0002-9637
DOI: 10.4269/ajtmh.17-0003
期刊: AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
Volume: 97
起始頁: 1049
結束頁: 1061
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