標題: | A hydrogen sulfide triggered self-immolative fluorescent probe for lysosome labeling in live cells |
作者: | Velusamy, Nithya Thirumalaivasan, Natesan Bobba, Kondapa Naidu Wu, Shu-Pao Bhuniya, Sankarprasad 應用化學系 Department of Applied Chemistry |
公開日期: | 7-二月-2018 |
摘要: | We developed a naphthalimide-based, lysosome-targeting, and self-immolative fluorescent probe for H2S detection. Probe LS-1 comprises a 2-formylbenzoate derivative of the 1,8-naphthalimide fluorophore. H2S forms a thiohemiacetal intermediate by reacting with the formyl group of the 2-formylbenzoate derivative, which subsequently undergoes intramolecular cyclization with the ester moiety to generate a free naphthalimide fluorophore (FL-1). The UV-vis absorbance (lambda(abs)) value of probe LS-1 at 450 nm increased in the presence of H2S. Similarly, with increasing H2S concentrations, the emission band (lambda(em)) centered at 560 nm increased gradually. The probe was found to be highly sensitive and chemoselective towards H2S compared with other biological analytes. Probe LS-1 was nontoxic and very stable across the physiological pH range. The probe LS-1 enables the detection of intracellular endogenous H2S formation in HT29 cells in lysosomes. |
URI: | http://dx.doi.org/10.1039/c7nj04119e http://hdl.handle.net/11536/144448 |
ISSN: | 1144-0546 |
DOI: | 10.1039/c7nj04119e |
期刊: | NEW JOURNAL OF CHEMISTRY |
Volume: | 42 |
起始頁: | 1590 |
結束頁: | 1594 |
顯示於類別: | 期刊論文 |