標題: Functionalized gold nanoparticles as affinity nanoprobes for multiple lectins
作者: Selvaprakash, Karuppuchamy
Chen, Yu-Chie
應用化學系
Department of Applied Chemistry
關鍵字: Lectins;Concanavalin A;Banana lectin;Ricin;Chicken ovalbumin
公開日期: 1-二月-2018
摘要: Glycan-lectin interactions are commonly observed in nature. Analytical methods, which are used to detect lectins that rely on the use of glycan ligand-modified nanoprobes as affinity probes, have been developed. Most of the existing methods are focused on the use of synthetic glycan ligands. Nevertheless, naturally available glycoproteins, such as ovalbumin in chicken egg whites, are good sources for fabricating glycan-immobilized nanoprobes. In this study, we generated functionalized gold nanoparticles (Au NPs) from a one-pot reaction by reacting chicken egg white (cew) proteins with aqueous tetrachloroaurate. The generated Au@cew NPs are mainly encapsulated by ovalbumin, in which the surface is decorated by abundant hybrid mannose and Gal beta(1 -> 4)GIcNAc-terminated glycan ligands. Thus, the generated Au@cew NPs containing hybrid mannose and Gal beta(1 -> 4)GIcNAc have the capability to selectively bind with their corresponding lectins. Lectins including concanavalin A, banana lectin, and ricin B that have binding moieties toward specific sugars were used as the model samples. Our results showed that the generated AuNPs can be used as multiplex affinity probes for these model lectins. Lectins can be selectively released from the Au@cew NP-lectin conjugates by using specific sugars, such as mannose, glucose, and beta-lactose, as the releasing agents to release specific lectins from the conjugates. Matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used as the tool to characterize the released species from the nanoprobes. The limit of detection of these model lectins using the current approach was low (in nM). The feasibility of using the Au@cew NP-based affinity MALDI-MS to selectively detect specific lectins from complex samples was also demonstrated. (C) 2017 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.colsurfb.2017.11.022
http://hdl.handle.net/11536/144527
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2017.11.022
期刊: COLLOIDS AND SURFACES B-BIOINTERFACES
Volume: 162
起始頁: 60
結束頁: 68
顯示於類別:期刊論文