標題: Instillation of particulate matter 2.5 induced acute lung injury and attenuated the injury recovery in ACE2 knockout mice
作者: Lin, Chung-I
Tsai, Chin-Hung
Sun, Yu-Ling
Hsieh, Wen-Yeh
Lin, Chang
Chen, Cheng-Yi
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: particulate matter 2.5;renin-angiotensin system;angiotensin-converting enzyme II;inflammation;acute lung injury
公開日期: 1-一月-2018
摘要: Inhaled particulate matter 2.5 (PM2.5) can cause lung injury by inducing serious inflammation in lung tissue. Renin-angiotensin system (RAS) is involved in the pathogenesis of inflammatory lung diseases and regulates inflammatory response. Angiotensin-converting enzyme II (ACE2), which is produced through the angiotensin-converting enzyme (ACE)/angiotensin II (Ang II) axis, protects against lung disease. However, few studies have focused on the relationships between PM2.5 and ACE2. Therefore, we aimed to explore the role of ACE2 in PM2.5-induced acute lung injury (ALI). An animal model of PM(2.)5-induced ALI was established with wild type (C57BL/6, WT) and ACE2 gene knockout (ACE2 KO) mice. The mice were exposed to PM2.5 through intratracheal instillation once a day for 3 days (6.25 mg/kg/day) and then sacrificed at 2 days and 5 days after PM2.5 instillation. The results show that resting respiratory rate (RRR), levels of inflammatory cytokines, ACE and MMPs in the lungs of WT and ACE2 KO mice were significantly increased at 2 days postinstillation. At 5 days postinstillation, the PM2.5-induced ALI significantly recovered in the WT mice, but only partially recovered in the ACE2 KO mice. The results hint that PM(2.)5 could induce severe ALI through pulmonary inflammation, and the repair after acute PM2.5 postinstillation could be attenuated in the absence of ACE2. Additionally, our results show that PM2.5-induced ALI is associated with signaling p-ERK1/2 and p-STAT3 pathways and ACE2 knockdown could increase pulmonary p-STAT3 and p-ERK1/2 levels in the PM(2.)5-induced ALI.
URI: http://dx.doi.org/10.7150/ijbs.23489
http://hdl.handle.net/11536/144572
ISSN: 1449-2288
DOI: 10.7150/ijbs.23489
期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
Volume: 14
起始頁: 253
結束頁: 265
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