完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yuyama, Ken-ichi | en_US |
dc.contributor.author | Chang, Kai-Di | en_US |
dc.contributor.author | Tu, Jing-Ru | en_US |
dc.contributor.author | Masuhara, Hiroshi | en_US |
dc.contributor.author | Sugiyama, Teruki | en_US |
dc.date.accessioned | 2018-08-21T05:53:31Z | - |
dc.date.available | 2018-08-21T05:53:31Z | - |
dc.date.issued | 2018-03-07 | en_US |
dc.identifier.issn | 1463-9076 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1039/c7cp06990a | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/144802 | - |
dc.description.abstract | Confining protein crystallization to a millimetre size was achieved within 0.5 h after stopping 1 h intense trapping laser irradiation, which shows excellent performance in spatial and temporal controllability compared to spontaneous nucleation. A continuous-wave near-infrared laser beam is tightly focused into a glass/solution interfacial layer of a supersaturated buffer solution of hen egg-white lysozyme (HEWL). The crystallization is not observed during laser trapping, but initiated by stopping the laser irradiation. The generated crystals are localized densely in a circular area with a diameter of a few millimetres around the focal spot and show specific directions of the optical axes of the HEWL crystals. To interpret this unique crystallization, we propose a mechanism that nucleation and the subsequent growth take place in a highly concentrated domain consisting of HEWL liquid-like clusters after turning off laser trapping. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Rapid localized crystallization of lysozyme by laser trapping | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1039/c7cp06990a | en_US |
dc.identifier.journal | PHYSICAL CHEMISTRY CHEMICAL PHYSICS | en_US |
dc.citation.volume | 20 | en_US |
dc.citation.spage | 6034 | en_US |
dc.citation.epage | 6039 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.identifier.wosnumber | WOS:000429280100003 | en_US |
顯示於類別: | 期刊論文 |