標題: Nanochip-Induced Epithelial-to-Mesenchymal Transition: Impact of Physical Microenvironment on Cancer Metastasis
作者: Dhawan, Udesh
Sue, Ming-Wen
Lan, Kuan-Chun
Buddhakosai, Waradee
Pao Hui Huang
Chen, Yi Cheng
Chen, Po-Chun
Chen, Wen Liang
材料科學與工程學系
生物科技學系
Department of Materials Science and Engineering
Department of Biological Science and Technology
關鍵字: epithelial to mesenchymal transition;nanotopography;artificial microenvironments;tantalum oxide;triple negative breast cancer;nanodots
公開日期: 11-四月-2018
摘要: Epithelial-to-mesenchymal transition (EMT) is a highly orchestrated process motivated by the nature of physical and chemical compositions of the tumor microenvironment (TME). The role of the physical framework of the TME in guiding cells toward EMT is poorly understood. To investigate this, breast cancer MDA-MB-231 and MCF-7 cells were cultured on nanochips comprising tantalum oxide nanodots ranging in diameter from 10 to 200 nm, fabricated through electrochemical approach and collectively referred to as artificial microenvironments. The 100 and 200 nm nanochips induced the cells to adopt an elongated or spindle-shaped morphology. The key EMT genes, E-cadherin, N-cadherin, and vimentin, displayed the spatial control exhibited by the artificial microenvironments. The E-cadherin gene expression was attenuated, whereas those of N-cadherin and vimentin were amplified by 100 and 200 rim nanochips, indicating the induction of EMT. Transcription factors, snail and twist, were identified for modulating the EMT genes in the cells on these artificial microenvironments. Localization of EMT proteins observed through immunostaining indicated the loss of cell cell junctions on 100 and 200 rim nanochips, confirming the EMT induction. Thus, by utilizing an in vitro approach, we demonstrate how the physical framework of the TME may possibly trigger or assist in inducing EMT in vivo. Applications in the fields of drug discovery, biomedical engineering, and cancer research are expected.
URI: http://dx.doi.org/10.1021/acsami.7b19467
http://hdl.handle.net/11536/144851
ISSN: 1944-8244
DOI: 10.1021/acsami.7b19467
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 10
起始頁: 11474
結束頁: 11485
顯示於類別:期刊論文