標題: Enhanced Osteogenic Differentiation of Stem Cells on Phase-Engineered Graphene Oxide
作者: Yang, Jia-Wei
Hsieh, Kuan Yu
Kumar, Priyank V.
Cheng, Sheng-Jen
Lin, You-Rong
Shen, Yu-Chih
Chen, Guan-Yu
分子醫學與生物工程研究所
資訊工程學系
生醫工程研究所
Institute of Molecular Medicine and Bioengineering
Department of Computer Science
Institute of Biomedical Engineering
關鍵字: graphene oxide;phase transformation;human mesenchymal stem cells;osteogenic peptide;cell differentiation
公開日期: 18-四月-2018
摘要: Graphene oxide (GO) has attracted significant interest as a template material for multiple applications due to its two-dimensional nature and established functionalization chemistries. However, for applications toward stem cell culture and differentiation, GO is often reduced to form reduced graphene oxide, resulting in a loss of oxygen content. Here, we induce a phase transformation in GO and demonstrate its benefits for enhanced stem cell culture and differentiation while conserving the oxygen content. The transformation results in the clustering of oxygen atoms on the GO surface, which greatly improves its ability toward substance adherence and results in enhanced differentiation of human mesenchymal stem cells toward the osteogenic lineage. Moreover, the conjugating ability of modified GO strengthened, which was examined by auxiliary osteogenic growth peptide conjugation. Overall, our work demonstrates GO's potential for stem cell applications while maintaining its oxygen content, which could enable further functionalization and fabrication of novel nano-biointerfaces.
URI: http://dx.doi.org/10.1021/acsami.8b02225
http://hdl.handle.net/11536/144890
ISSN: 1944-8244
DOI: 10.1021/acsami.8b02225
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 10
起始頁: 12497
結束頁: 12503
顯示於類別:期刊論文