標題: Constrained Adherable Area of Nanotopographic Surfaces Promotes Cell Migration through the Regulation of Focal Adhesion via Focal Adhesion Kinase/Racl Activation
作者: Lim, Jiwon
Choi, Andrew
Kim, Hyung Woo
Yoon, Hyungjun
Park, Sang Min
Tsai, Chia-Hung Dylan
Kaneko, Makoto
Kim, Dong Sung
機械工程學系
Department of Mechanical Engineering
關鍵字: adherable area;polystyrene nanopore surface;fibroblast cell migration;focal adhesion;FAK;Racl
公開日期: 2-May-2018
摘要: Cell migration is crucial in physiological and pathological processes such as embryonic development and wound healing; such migration is strongly guided by the surrounding nanostructured extracellular matrix Previous studies have extensively studied the cell migration on anisotropic nanotopographic surfaces; however, only a few studies have reported cell migration on isotropic nanotopographic surfaces. We herein, for the first time, propose a novel concept of adherable area on cell migration using isotropic nanopore surfaces with sufficient nanopore depth by adopting a high aspect ratio. As the pore size of the nanopore surface was controlled to 200, 300, and 400 nm in a fixed center-to-center distance of 480 nm, it produced 86, 68, and 36% of adherable area, respectively, on the fabricated surface. A meticulous investigation of the cell migration in response to changes in the constrained adherable area of the nanotopographic surface showed 1.4-, 1.5-, and 1.6-fold increase in migration speeds and a 1.4-, 2-, and 2.5-fold decrease in the number of focal adhesions as the adherable area was decreased to 86, 68, and 36%, respectively. Furthermore, a strong activation of FAK/Racl signaling was observed to be involved in the promoted cell migration. These results suggest that the reduced adherable area promotes cell migration through decreasing the FA formation, which in turn upregulates FAK/Racl activation. The findings in this study can be utilized to control the cell migration behaviors, which is a powerful tool in the research fields involving cell migration such as promoting wound healing and tissue repair.
URI: http://dx.doi.org/10.1021/acsami.7b18954
http://hdl.handle.net/11536/144956
ISSN: 1944-8244
DOI: 10.1021/acsami.7b18954
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 10
起始頁: 14331
結束頁: 14341
Appears in Collections:Articles