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dc.contributor.authorHuang, Bo-Yuen_US
dc.contributor.authorChen, Po-Chungen_US
dc.contributor.authorChen, Bo-Hanen_US
dc.contributor.authorWang, Chen-Chuen_US
dc.contributor.authorLiu, Hsuan-Fuen_US
dc.contributor.authorChen, Yi-Zaoen_US
dc.contributor.authorChen, Chien-Shengen_US
dc.contributor.authorYang, Yuh-Shyongen_US
dc.date.accessioned2018-08-21T05:53:46Z-
dc.date.available2018-08-21T05:53:46Z-
dc.date.issued2017-03-21en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/acs.analchem.6b02853en_US
dc.identifier.urihttp://hdl.handle.net/11536/145120-
dc.description.abstractProtein tyrosine sulfation (PTS) is a widespread posttranslational modification that induces intercellular and extracellular responses by regulating protein protein interactions and enzymatic activity. Although PTS affects numerous physiological and pathological processes, only a small fraction of the total predicted sulfated proteins has been identified to date. Here, we localized the potential sulfation sites of Escherichia coli proteins on a proteome microarray by using a 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase-coupled tyrosylprotein sulfotransferase (TPST) catalysis system that involves in situ PAPS generation and TPST catalysis. Among the 4256 E. coil K12 proteins, 875 sulfated proteins were identified using antisulfotyrosine primary and Cy3-labeled antimouse secondary antibodies. Our findings add considerably to the list of potential proteins subjected to tyrosine sulfation. Similar procedures can be applied to identify sulfated proteins in yeast and human proteome microarrays, and we expect such approaches to contribute substantially to the understanding of important human diseases.en_US
dc.language.isoen_USen_US
dc.titleHigh-Throughput Screening of Sulfated Proteins by Using a Genome-Wide Proteome Microarray and Protein Tyrosine Sulfation Systemen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/acs.analchem.6b02853en_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume89en_US
dc.citation.spage3278en_US
dc.citation.epage3284en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000397478300009en_US
Appears in Collections:Articles